Journal of Advances in Technology and Engineering Research
Details
Journal ISSN: 2414-4592
Article DOI: https://doi.org/10.20474/jater-2.4.4
Received: 19 January 2016
Accepted: 12 July 2016
Published: 12 August 2016
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  • Effects of different plant growth hormones on in vitro regeneration of a new Malaysian rice variety (Oryza Sativa L. Var. Mria 1) from stem explants

Nor Yasmin M. F., Norrizah J. S., Azani S.

Published online: 2016

Abstract

A study was conducted to determine the effects of different plant growth hormones towards in vitro regeneration of a new Malaysian rice variety (Oryza sativa L.Var. MRIA 1). Stem explants of 4-week- old aseptic seedlings were selected and a total of 16 different combinations of NAA, IBA, BAP, and Kn were utilised. The most suitable plant growth hormones for in vitro regeneration of Oryza sativa L. Var. MRIA 1 was obtained from MS (Murashige and Skoog) media supplemented with 1.0 mg/l NAA and 0.5 mg/l Kn with an average of 8.90 ± 1.79 number of shoots, 114.00 ± 18.90 number of roots, 10.70 ± 2.62 cm length of shoots and 8.70 ± 1.64 number of leaves per explant. It was proved that the in vitro regeneration of Oryza sativa L. Var. MRIA 1 from stem explants was achieved without entering the intermediate callus phase within 4 weeks of culture. Thus, laborious sub-culturing processes were not required to regenerate the multiple shoots. These findings provided an optimum plant growth hormone concentration for the tissue culture system of Oryza sativa L. Var. MRIA 1. Acclimatization of regenerated plantlets of Oryza sativa L. Var. MRIA 1 is recommended in order to serve a complete in vitro regeneration protocol for the variety.