Journal of Applied and Physical Sciences
Details
Journal ISSN: 2414-3103
Artical DOI: DOI:https://doi.org/10.20474/japs-1.1.2
Received: 31 October 2014
Accepted: 16 August, 2015
Published: 15 October 2015
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  • The effects of picloram and 2,4-dichlorophenoxyacetic acid on the induction of red colored callus from Celosia plumose as an attractive ornamental plant

Noraini Mahmad, Rosna Mat Taha, Norlina Rawi. Sadegh Mohajer

Article first published online: 2015

Abstract

Celosia plumosa is an attractive ornamental plant, which has flowers described as having a flame-like color and is very useful for being used as a traditional medicinal herbs. The current study deals with the induction of red-colored callus from root, stem and leaf explants when they are cultured on a Murashige and Skoog (MS) medium supplemented with different concentrations, which are separately applied, of auxin picloram and 2,4-dichlorophenoxyacetic acid (2,4-D). For the culturing on MS medium supplemented with 6.0 mg/l picloram, the highest amount of red-colored callus (0.49±0.26 g) appears to result from leaf explants, while the lowest (0.09±0.02 g) comes from stem explants. However, the highest amount of red-colored callus (0.69±0.13 g) achieved on MS medium supplemented with 1.5 mg/l 2,4-D is produced by stem explants, while the lowest (0.13±0.06 g) comes from root explants, which is cultured on 0.5 mg/l 2,4-D. In general, the optimum concentration for red-colored callus formation using picloram (6.0 mg/l) is higher than 2,4-D (1.5 mg/l 2,4-D).