Journal of Applied and Physical Sciences
Journal ISSN: 2414-3103
Article DOI:
Received: 14 January 2017
Accepted: 19 January 2017
Published: 30 June 2017
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  • Isolation and selection of purple non-sulfur bacteria for phosphate removal in rearing water from shrimp cultivation

Patama Bunruk, Duangporn Kantachote, Ampaitip Sukhoom

Article first published online: 2017


One of the key environmental concerns about shrimp cultivation is the discharge of rearing water with high levels of nutrients, especially phosphate into waterways, resulting in eutrophication. To solve this problem, biological treatment is well-recognized and the use of purple non-sulfur bacteria (PNSB) is one of alternative attractive choices because of their high removal efϐiciency inwastewater treatmentwith various metabolic growth conditions. Therefore, this study aimed to isolate and screen PNSB from shrimp ponds with their ability for reducing phosphate in water from shrimp cultivation. A total of 83 PNSB strains were isolated from water and sediment samples collected from various 15 shrimp ponds located in Phangnga and Songkhla provinces. For primary screening, there were 42 strains (51%) that grew well (OD660> 1.0) in glutamate-acetate broth supplemented with 1.5% (w/v) NaCl, under conditions of microaerobiclight and aerobic-dark. However, in secondary screening only two strains (W12 and W48) could grow in sterile rearing water collected from shrimp pond. They were selected for tertiary screening to investigate their ability to remove phosphate in sterile rearing water under both incubating conditions. Both PNSB strains produced no signiϐicant differences for phosphate removal efϐiciency (> 50%) with exception under microaerobic-light conditions as strain W12 roughly reduced 46% phosphate. Of these, 2 strains had the possibility to be used as inoculants for removal of phosphate from rearing water in shrimp ponds